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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- H00000435-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000435-M01, RRID:AB_425316
- Product name
- ASL monoclonal antibody (M01), clone 4C5-1F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ASL.
- Antigen sequence
MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLW
EVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKV
AEEWAQGTFKLNSNDEDIHTANERRLKELIGATAG
KLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELI
RTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILS
HAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLG
VDRELLRAELNFGAITLNSMDATSERDFVAELLFW
ASLCMTHLSRMAEDLILYCTKEFSFVQPSDAYSTG
SSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLK
GLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVI
STLQIHQENMGQALSPDMLATDLAYYLVRKGMPFR
QAHEASGKAVFMAETKGVALNQLSLQELQTISPLF
SGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQV
RALLQAQQA- Isotype
- IgG
- Antibody clone number
- 4C5-1F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Epigenetic status of argininosuccinate synthetase and argininosuccinate lyase modulates autophagy and cell death in glioblastoma.
Syed N, Langer J, Janczar K, Singh P, Lo Nigro C, Lattanzio L, Coley HM, Hatzimichael E, Bomalaski J, Szlosarek P, Awad M, O'Neil K, Roncaroli F, Crook T
Cell death & disease 2013 Jan 17;4:e458
Cell death & disease 2013 Jan 17;4:e458
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ASL expression in transfected 293T cell line by ASL monoclonal antibody (M01), clone 4C5-1F2.Lane 1: ASL transfected lysate(51.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ASL on HeLa cell. [antibody concentration 5 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
