Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB751-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse VEGF-B167/186 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse VEGF-B in direct ELISAs and Western blots. Recognizes both the VEGF-B167 and VEGF-B186 isoforms. In Western blots, no cross-reactivity with recombinant human (rh) VEGF, rhVEGF-C, rhVEGF-D, rhCTGF, rhPlGF, rhLDGF, or rhPDGF is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAB06274
- Isotype
- IgG
- Antibody clone number
- 58013
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Correlation of Vascular Endothelial Growth Factor subtypes and their receptors with melanoma progression: A next-generation Tissue Microarray (ngTMA) automated analysis.
Seyed Jafari SM, Wiedmer C, Cazzaniga S, Frangež Ž, Shafighi M, Beltraminelli H, Weber B, Simon HU, Hunger RE
PloS one 2018;13(11):e0207019
PloS one 2018;13(11):e0207019
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGF-B167/186 in Human Breast Cancer Tissue. VEGF-B167/186 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse VEGF-B167/186 Monoclonal Antibody (Catalog # MAB751) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.