LS-C186115

antibody from LSBio

Targeting: VIL1 D2S1471, VIL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C186115

Provider

LSBio

Product name

VIL1 / Villin Antibody (aa7-246) LS-C186115

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. Villin antibody diluted at 1:5000

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Villin antibody detects VIL1 protein by Western blot analysis. A. 50 ug mouse kidney lysate/extract. 7.5 % SDS-PAGE. Villin antibody dilution:1:1000

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Villin antibody detects VIL1 protein by Western blot analysis. A. 50 ug rat kidney lysate/extract. 7.5 % SDS-PAGE. Villin antibody dilution:1:2000

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa using Villin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded A549 xenograft using Villin antibody at 1:500 dilution.