Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9457 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Villin/VIL1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for VILLIN/VIL1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. VILLIN/VIL1 information: Molecular Weight: 92695 MW; Subcellular Localization: Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment; Tissue Specificity: Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora.
Acid and bile salt up-regulate BMP4 expression in human esophageal epithelium cells.
Zhang X, Tong Y, Lyu X, Wang J, Wang Y, Yang R
Frontiers in microbiology 2020;11:622354
Frontiers in microbiology 2020;11:622354
Acid and bile salt up-regulate BMP4 expression in human esophageal epithelium cells.
Zhou G, Sun YG, Wang HB, Wang WQ, Wang XW, Fang DC
Scandinavian journal of gastroenterology 2009;44(8):926-32
Scandinavian journal of gastroenterology 2009;44(8):926-32
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti- Villin Picoband antibody, PB9457, Western blottingAll lanes: Anti Villin (PB9457) at 0.5ug/mlLane 1: Rat Intestine Tissue Lysate at 50ugLane 2: Mouse Kidney Tissue Lysate at 50ugLane 3: RH35 Whole Cell Lysate at 40ugLane 4: HEPG2 Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 93KDObserved bind size: 93KD
- Additional image