H00007429-M26

antibody from Abnova Corporation

Targeting: VIL1 D2S1471, VIL

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007429-M26

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007429-M26, RRID:AB_1716978

Product name

VIL1 monoclonal antibody (M26), clone 2F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant VIL1.

Antigen sequence

MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSS
TFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDS
SLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESE
AFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLL
HVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLII
QWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGV
VDGENELASPKLMEVMNHVLGKRRELKAAVPDTVV
EPALKAALKLYHVSDSEGNLVVREVATRPLTQDLL
SHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHA
LNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT
ASNRTSGLGKTHTVGSVGEGQAGAVREPGSRSWAR
RATWSTTHPPSLTCIFNEDFYAGSGLVLADGDVDK
L


Isotype

IgG

Antibody clone number

2F10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of VIL1 expression in transfected 293T cell line by VIL1 monoclonal antibody (M26), clone 2F10.Lane 1: VIL1 transfected lysate(46.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged VIL1 is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol