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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-30743 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLE3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, POLE3 shRNA-transfected 293T.
- Concentration
- 1.01 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using POLE3 Polyclonal Antibody (Product # PA5-30743). Sample (30 µg of whole cell lysate). Lane A: 293T . 15% SDS PAGE. POLE3 Polyclonal Antibody (Product # PA5-30743) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNA Polymerase epsilon in paraformaldehyde-fixed HeLa cells using a DNA Polymerase epsilon polyclonal antibody (Product # PA5-30743) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNA polymerase epsilon antibody [N1C3] detects DNA Polymerase epsilon protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. DNA Polymerase epsilon antibody [N1C3] (Product # PA5-30743) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DNA polymerase epsilon (Product # PA5-30743) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
