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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-89462 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NXT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GLDALNNFFD TLPSSEFQVN MLDCQPVHEQ ATQSQTTVLV VTSGTVKFDG NKQHFFNQNF LLTAQSTPNN T; Positive Samples: Mouse testis, Rat testis; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.02 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NXT2 in extracts of various cell lines using NXT2 Polyclonal Antibody (Product # PA5-89462) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NXT2 was performed in L929 cells using NXT2 Polyclonal Antibody (Product # PA5-89462) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NXT2 was performed in U-2 OS cells using NXT2 Polyclonal Antibody (Product # PA5-89462) at a dilution of 1:100. Blue: DAPI for nuclear staining.
