PAB2968

antibody from Abnova Corporation

Targeting: HARS1 HARS, HisRS, USH3B

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2968

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2968, RRID:AB_1575793

Product name

HARS polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of HARS.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Novel conformation of histidyl-transfer RNA synthetase in the lung: the target tissue in Jo-1 autoantibody-associated myositis.

Levine SM, Raben N, Xie D, Askin FB, Tuder R, Mullins M, Rosen A, Casciola-Rosen LA
Arthritis and rheumatism 2007 Aug;56(8):2729-39

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TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation.

Lu Q, Hope LW, Brasch M, Reinhard C, Cohen SN
Proceedings of the National Academy of Sciences of the United States of America 2003 Jun 24;100(13):7626-31

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of HARS polyclonal antibody (Cat # PAB2968) in K-562 cell line lysates (35 ug/lane).HARS (arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with HARS polyclonal antibody (Cat # PAB2968), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)