MA3-16726

antibody from Invitrogen Antibodies

Targeting: INA NEF5, NF-66

Provider product page for MA3-16726

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [6]
Comments [0]
Validations
Western blot [3]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: MA3-16726 - Provider product page
Provider: Invitrogen Antibodies
Product name: alpha Internexin Monoclonal Antibody (2E3)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: The epitope recognized by the 2E3 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so this antibody is ideal for studies of formalin-fixed paraffin-embedded histological sections.
Reactivity: Human, Mouse, Rat, Bovine, Porcine
Host: Mouse
Isotype: IgG
Antibody clone number: 2E3
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

INA protein structure - MA3-16726 shown in red.

Submitted references TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis.

van der Zee J, Gijselinck I, Van Mossevelde S, Perrone F, Dillen L, Heeman B, Bäumer V, Engelborghs S, De Bleecker J, Baets J, Gelpi E, Rojas-García R, Clarimón J, Lleó A, Diehl-Schmid J, Alexopoulos P, Perneczky R, Synofzik M, Just J, Schöls L, Graff C, Thonberg H, Borroni B, Padovani A, Jordanova A, Sarafov S, Tournev I, de Mendonça A, Miltenberger-Miltényi G, Simões do Couto F, Ramirez A, Jessen F, Heneka MT, Gómez-Tortosa E, Danek A, Cras P, Vandenberghe R, De Jonghe P, De Deyn PP, Sleegers K, Cruts M, Van Broeckhoven C, Goeman J, Nuytten D, Smets K, Robberecht W, Damme PV, Bleecker J, Santens P, Dermaut B, Versijpt J, Michotte A, Ivanoiu A, Deryck O, Bergmans B, Delbeck J, Bruyland M, Willems C, Salmon E, Pastor P, Ortega-Cubero S, Benussi L, Ghidoni R, Binetti G, Hernández I, Boada M, Ruiz A, Sorbi S, Nacmias B, Bagnoli S, Sorbi S, Sanchez-Valle R, Llado A, Santana I, Rosário Almeida M, Frisoni GB, Maetzler W, Matej R, Fraidakis MJ, Kovacs GG, Fabrizi GM, Testi S
Human mutation 2017 Mar;38(3):297-309

Neuropathological criteria of anti-IgLON5-related tauopathy.

Gelpi E, Höftberger R, Graus F, Ling H, Holton JL, Dawson T, Popovic M, Pretnar-Oblak J, Högl B, Schmutzhard E, Poewe W, Ricken G, Santamaria J, Dalmau J, Budka H, Revesz T, Kovacs GG
Acta neuropathologica 2016 Oct;132(4):531-43

An autopsy case of neuronal intermediate filament inclusion disease with regard to immunophenotypic and topographical analysis of the neuronal inclusions.

Inoue K, Fujimura H, Ueda K, Matsumura T, Itoh K, Sakoda S
Neuropathology : official journal of the Japanese Society of Neuropathology 2015 Dec;35(6):545-52

Cytoplasmic non-epithelial mucin accumulation associated with CD44 in an astrocytic tumor with signet ring features.

Okabe H, Nagata A, Katsura K, Ishida M, Osaka Y, Tenjin H
Brain tumor pathology 2014 Apr;31(2):124-30

Gliosarcoma with ependymal and PNET-like differentiation.

Shintaku M, Yoneda H, Hirato J, Nagaishi M, Okabe H
Clinical neuropathology 2013 Nov-Dec;32(6):508-14

Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.

Shaw G, Morse S, Ararat M, Graham FL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jun;16(8):869-71

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of homogenate of rat facial nucleus incubated with MA3-16726, which reveals a single clean band running at approx. 66kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Internexin in different tissue lysates. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16726 using a dilution of 1:10000. Antibody in red: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord lysate. The antibody reveals the alpha-Internexin protein with an apparent molecular weight of 64-66 kDa, with some variability across species.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of alpha Internexin in 0.5 mg/mL SH-SY5Y lysate. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16726). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of alpha Internexin in Hippocampal neurons in tissue culture. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16726). Neurofilament alpha internexin (red), using alpha-Internexin Antibody and GFAP antibody (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of alpha Internexin in a section of rat facial nucleus 7 days following axotomy. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16726). These neurons are capable of regenerating their axons and also, concomitant with regeneration, strongly upregulate alpha-Internexin in their perikarya. Other central neurons which are not able to regenerate their axons do not upregulate this protein after axotomy and untreated facial neurons normally show only very low levels of alpha-Internexin. Both findings suggest that alpha-Internexin has a role in axonal regeneration.