PA5-97931

antibody from Invitrogen Antibodies

Targeting: LPAR2 EDG-4, EDG4, LPA2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97931

Provider

Invitrogen Antibodies

Product name

EDG4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

4.205 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EDG4 using a EDG4 Polyclonal antibody (Product # PA5-97931). Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, COLO205 whole cell lysate, PC-3 whole cell lysate. All lanes: LPAR2 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 40 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EDG4 using a EDG4 Polyclonal antibody (Product # PA5-97931). Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, COLO205 whole cell lysate, PC-3 whole cell lysate. All lanes: LPAR2 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 40 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of EDG4 in paraffin embedded human prostate tissue using a EDG4 polyclonal antibody (Product # PA5-97931) at a dilution of 1:800. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.