15036-1-AP

antibody from Invitrogen Antibodies

Targeting: SPINT1 HAI, MANSC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15036-1-AP

Provider

Invitrogen Antibodies

Product name

HAI-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TLGLQGTQA GPPPAPPGLP AGADCLNSFT AGVPGFVLDT NASVSNGATF LESPTVRRGW DCVRACCTTQ NCNLALVELQ PDRGEDAIAA CFLINCLYEQ NFVCKFAPRE GFINYLTREV YRSYRQLRTQ GFGGSGIPKA WAGIDLKVQP QEPLVLKDVE NTDWRLLRGD TDVRVERKDP NQVELWGLKE GTYLFQLTVT SSDHPEDTAN VTVTVLSTKQ TEDYCLASNK VGRCRGSFPR WYYDPTEQIC KSFVYGGCLG NKNNYLREEE CILAC (27-300 aa encoded by B C004140)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 15036-1-AP ( HAI-1 antibody at dilution of 1:1000 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 15036-1-AP ( HAI-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 15036-1-AP (HAI-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP ( HAI-1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP ( HAI-1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).