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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-78263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p35 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: HeLa
- Concentration
- 0.97 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p35 in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with p35 polyclonal antibody (Product # PA5-78263) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of p35 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: p35 Polyclonal Antibody (Product # PA5-78263) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p35 was performed in paraffin-embedded mouse brain tissue using p35 Polyclonal Antibody (Product # PA5-78263) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p35 was performed in paraffin-embedded rat brain tissue using p35 Polyclonal Antibody (Product # PA5-78263) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
