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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-14834 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p35 Monoclonal Antibody (B.33.3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- B.33.3
- Vial size
- 100 µL
- Concentration
- 146 µg/mL
- Storage
- -20°C
Submitted references Abnormal Mitochondria in a Non-human Primate Model of MPTP-induced Parkinson's Disease: Drp1 and CDK5/p25 Signaling.
Park J, Seo J, Won J, Yeo HG, Ahn YJ, Kim K, Jin YB, Koo BS, Lim KS, Jeong KJ, Kang P, Lee HY, Baek SH, Jeon CY, Hong JJ, Huh JW, Kim YH, Park SJ, Kim SU, Lee DS, Lee SR, Lee Y
Experimental neurobiology 2019 Jun;28(3):414-424
Experimental neurobiology 2019 Jun;28(3):414-424
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HepG2 (Lane 2), HT-29 (Lane 3), U-2 OS (Lane 4) and PANC-1 (Lane 5). The blot was probed with p35 Monoclonal Antibody (B.33.3) (Product # MA5-14834, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 35kDa band corresponding to p35 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p35 using a monoclonal antibody (Product # MA5-14834).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p35/25 in mouse cerebellum using a p35/25 monoclonal antibody (Product # MA5-14834) (green). DNA is labeled using a fluorescent blue dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of p35/25 in paraffin-embedded human meningioma using a p35/25 monoclonal antibody (Product # MA5-14834).
