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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- GTX103261 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103261, RRID:AB_1949798
- Product name
- Calretinin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) 12% SDS PAGE Calretinin antibody GTX103261 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX103261 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Calretinin antibody detects CALB2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human CALB2-transfected 293T cells12% SDS-PAGECalretinin antibody (GTX103261) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin (GTX103261) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin(GTX103261) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Calretinin antibody (GTX103261) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calretinin antibody detects Calretinin protein by immunohistochemical analysis.Samples: Frozen Section adult mouse hippocampus.Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
