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LearnTA324544
antibody from OriGene
Targeting: PITX2
ARP1, Brx1, IGDS, IHG2, IRID2, Otlx2, RGS, RIEG, RIEG1, RS
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA324544 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal PITX2 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal PITX2 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PITX2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.43 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Pilx2 Pab (Cat.#TA324544) in CEM cell line lysates (35ug/lane). Pilx2(arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with PITX2 Antibody (C-term)(Cat#TA324544/SA070209B).Hela cells were incubated with PITX2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).PITX2 immunoreactivity is localized to Nucleus significantly.
- Validation comment
- IF
