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antibody from Invitrogen Antibodies
Targeting: PITX2
ARP1, Brx1, IGDS, IHG2, IRID2, Otlx2, RGS, RIEG, RIEG1, RS
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-98817 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PITX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references Perturbation of Wnt/β-catenin signaling and sexual dimorphism in non-alcoholic fatty liver disease.
Yeh MM, Shi X, Yang J, Li M, Fung KM, Daoud SS
Hepatology research : the official journal of the Japan Society of Hepatology 2022 May;52(5):433-448
Hepatology research : the official journal of the Japan Society of Hepatology 2022 May;52(5):433-448
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PITX2 using a PITX2 Polyclonal antibody (Product # PA5-98817) at a concentration of 3 µg/mL. Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PITX2 using a PITX2 Polyclonal antibody (Product # PA5-98817) at a concentration of 3 µg/mL. Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 42 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PITX2 in Hela cells using a PITX2 polyclonal antibody (Product # PA5-98817) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PITX2 in paraffin embedded human duodenum tissue using a PITX2 polyclonal antibody (Product # PA5-98817) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PITX2 in paraffin embedded human small intestine tissue using a PITX2 polyclonal antibody (Product # PA5-98817) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 FIGURE DEGs between male and female cases in NAFLD using Wnt focusing RT 2 profiler Polymerase chain reaction (PCR) arrays. (a) Scatter plot comparing the normalized average expression of each gene of the ""Wnt targets"" PCR array between the two groups (cases 8 & 5 ""NAFLD"" vs. cases 2 & 3 ""normal"" in female samples). The red and green dot stand for up-regulated and down-regulated genes, respectively, whereas black dots indicate unchanged genes; cut-off = +-1.5. (b) Visualization of log2 (Fold Change). Two groups of differential genes in female samples, intersect and merge gene expression colorimetry maps. Based on the expression levels of the DEGs in each in groups, a log2 is taken. Using systematic clustering (Hierarchical Cluster), the overall clustering result of the sample is obtained. Red indicates higher expression and blue indicates lower expression. (c) Scatter plot comparing the normalized average expression of each gene of the ""Wnt targets"" PCR array between the two groups (cases 6 & 7 ""NAFLD"" vs. cases 1 & 4 ""normal"") in male samples. (d) Visualization of log2 (Fold Change) of gene expression in male samples. (e) Protein expression of beta-catenin, FRZB, PITX2 and WIF1 in normal and NASH samples analyzed by immunoblotting. The samples include Normal 1 (male), Normal 2 (female), NASH 1 (male) and NASH 2 (female). Primary antibodies against beta-catenin, FRZB, PITX2 and WIF1 were used for detection. beta-Actin was used as the loading (reference) control.
