CABT-B9280

antibody from Creative Diagnostics

Targeting: RPS6KB2 KLS, P70-BETA, p70S6Kb, S6KB, S6Kbeta, S6Kβ, STK14B

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9280

Provider

Creative Diagnostics

Product name

Mouse anti-Rat p70/S6K monoclonal antibody, clone 27/q80[t7l]

Antibody type

Monoclonal

Description

Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational components, such as ribosomal protein S6. p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6 phosphorylation, belongs to a family of at least six S6 kinases. p70 [S6K] and p85 [S6K] are two isoforms of the same kinase. Although they are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence. These kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic stimulation. Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389. p70 [S6K] associates with PDK-1 and PKCšø to form a possible PI3-K signaling complex. The immunosuppressive agent rapamycin forms an intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme activation. Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and proliferative responses. p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

27/q80[t7l]

Vial size

50ug, 150ug

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.