AF-456-NA

antibody from Novus Biologicals

Targeting: CCL7 FIC, MARC, MCP-3, MCP3, NC28, SCYA6, SCYA7

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-456-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal CCL7/MCP-3/MARC Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation.

He J, Song Y, Li G, Xiao P, Liu Y, Xue Y, Cao Q, Tu X, Pan T, Jiang Z, Cao X, Lai L, Wang Q
The Journal of clinical investigation 2019 Jun 27;129(9):3877-3893

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Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia.

Kwiatkowski K, Popiolek-Barczyk K, Piotrowska A, Rojewska E, Ciapała K, Makuch W, Mika J
Cytokine 2019 Jul;119:202-213

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CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection.

Girkin J, Hatchwell L, Foster P, Johnston SL, Bartlett N, Collison A, Mattes J
Journal of immunology (Baltimore, Md. : 1950) 2015 May 15;194(10):4924-30

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The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.

Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K, Ichijo H, Sawada Y, Sasaki K, Kobayashi T, Tamura S
The Journal of biological chemistry 2014 Mar 7;289(10):6438-6450

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Comprehensive assessment of chemokine expression profiles by flow cytometry.

Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23

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The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.

Szymczak WA, Deepe GS Jr
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 1;183(3):1964-74

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody. Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/ MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND₅₀ is typically 7.5-45 µg/mL.