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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- MAB320-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL11/Eotaxin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CCL11/Eotaxin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma , 14, 17, 19, 20, 21, 25, recombinant mouse CCL2, 3, 4, 5,6, 7, 9/10/MIP-1 gamma , 11, 21, or 25.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P51671- Isotype
- IgG
- Antibody clone number
- 43911
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Després S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
Nature 2011 Aug 31;477(7362):90-4
Nature 2011 Aug 31;477(7362):90-4
Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
Miyagaki T, Sugaya M, Fujita H, Ohmatsu H, Kakinuma T, Kadono T, Tamaki K, Sato S
The Journal of investigative dermatology 2010 Sep;130(9):2304-11
The Journal of investigative dermatology 2010 Sep;130(9):2304-11
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Osei-Kumah A, Smith R, Clifton VL
Cytokine 2008 Aug;43(2):187-93
Cytokine 2008 Aug;43(2):187-93
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
Hajnická V, Vancová I, Kocáková P, Slovák M, Gasperík J, Sláviková M, Hails RS, Labuda M, Nuttall PA
Parasitology 2005 Mar;130(Pt 3):333-42
Parasitology 2005 Mar;130(Pt 3):333-42
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Böttcher MF, Jenmalm MC, Björkstén B, Garofalo RP
Pediatric research 2000 May;47(5):592-7
Pediatric research 2000 May;47(5):592-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL11/Eotaxin in Human Skin. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL11/Eotaxin in Human Colon. CCL11/Eotaxin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial and stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCL11/Eotaxin Antibody. Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Goat Anti-Human CCL11/Eotaxin (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB320). The ND50 is typically 1-5 µg/mL.
