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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- 500-P41-50UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Eotaxin Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP.
- Concentration
- 0.1-1.0 mg/mL
Submitted references TARC and IL-5 expression correlates with tissue eosinophilia in peripheral T-cell lymphomas.
Degranulating eosinophils in human endometriosis.
Differential chemokine expression in tissues involved by Hodgkin's disease: direct correlation of eotaxin expression and tissue eosinophilia.
CD26/dipeptidyl-peptidase IV down-regulates the eosinophil chemotactic potency, but not the anti-HIV activity of human eotaxin by affecting its interaction with CC chemokine receptor 3.
Thielen C, Radermacher V, Trimeche M, Roufosse F, Goldman M, Boniver J, de Leval L
Leukemia research 2008 Sep;32(9):1431-8
Leukemia research 2008 Sep;32(9):1431-8
Degranulating eosinophils in human endometriosis.
Blumenthal RD, Samoszuk M, Taylor AP, Brown G, Alisauskas R, Goldenberg DM
The American journal of pathology 2000 May;156(5):1581-8
The American journal of pathology 2000 May;156(5):1581-8
Differential chemokine expression in tissues involved by Hodgkin's disease: direct correlation of eotaxin expression and tissue eosinophilia.
Teruya-Feldstein J, Jaffe ES, Burd PR, Kingma DW, Setsuda JE, Tosato G
Blood 1999 Apr 15;93(8):2463-70
Blood 1999 Apr 15;93(8):2463-70
CD26/dipeptidyl-peptidase IV down-regulates the eosinophil chemotactic potency, but not the anti-HIV activity of human eotaxin by affecting its interaction with CC chemokine receptor 3.
Struyf S, Proost P, Schols D, De Clercq E, Opdenakker G, Lenaerts JP, Detheux M, Parmentier M, De Meester I, Scharpé S, Van Damme J
Journal of immunology (Baltimore, Md. : 1950) 1999 Apr 15;162(8):4903-9
Journal of immunology (Baltimore, Md. : 1950) 1999 Apr 15;162(8):4903-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hEotaxin by Western Blot analysis Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect hEotaxin by Western Blot analysis Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human Eotaxin (CCL11) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of Eotaxin Polyclonal Antibody (Product # 500-P41-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech Eotaxin Polyclonal Antibody, Biotin (Product # 500-P41BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin (CCL11).
