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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- ABIN2508761 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Chemokine (C-C Motif) Ligand 8 (CCL8) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-2. Anti-Human MCP-2 specific antibody was purified by affinity chromatography employing immobilized hMCP-2 matrix.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- -20°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat lung lysate (lanes 3 and 6): 1-3. Anti-NKCC1 (extracellular) antibody (ABIN2511160), (1:200). 4-6. Anti-NKCC1 (extracellular) antibody, preincubated with the control peptide antigen. Expression of NKCC1 in rat cerebellum Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NKCC1 (extracellular) antibody (ABIN2511160), (1:100). A. NKCC1 staining (red) is detected in the cerebellar molecular layer (Mol). B. DAPI counterstain (blue) reveals the outline of cerebellar layers. C. Merge of the two images. Expression of NKCC1 in human U-87 MG cells Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells with Anti-NKCC1 (extracellular) antibody (ABIN2511160), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of the two pictures.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunocytochemistry
