Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF845 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL1/I-309/TCA-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human I-309 is observed and 2% cross-reactivity with recombinant mouse MCP-5 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P10146- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CCR8(+)FOXp3(+) T(reg) cells as master drivers of immune regulation.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
Barsheshet Y, Wildbaum G, Levy E, Vitenshtein A, Akinseye C, Griggs J, Lira SA, Karin N
Proceedings of the National Academy of Sciences of the United States of America 2017 Jun 6;114(23):6086-6091
Proceedings of the National Academy of Sciences of the United States of America 2017 Jun 6;114(23):6086-6091
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8576-84
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8576-84
IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8576-84
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 15;181(12):8576-84
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL1/ITCA-3 and Neutralization by Mouse CCL1/ITCA-3 Antibody. Recombinant Mouse CCL1/ TCA-3 (Catalog # 845-TC) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/ TCA-3 (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Mouse CCL1/TCA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF845). The ND50 is typically 0.3-1.5 µg/mL.
