NBP2-53178-100ug
antibody from Novus Biologicals
Targeting: S100A4
18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [5]
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Validation data
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- Product number
- NBP2-53178-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal S100A4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Western Blot of HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1481).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Western Blot Analysis of HeLa cell lysate using S100A4 Antibody (S100A4/1481).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: S100A4 Antibody (S100A4/1481) [NBP2-53178] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1481) NBP2-54580AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image from the Alexa Fluor 700 version of this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Formalin--paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1481).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): S100A4 Antibody (S100A4/1481) [NBP2-53178] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1481) NBP2-54580APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image from the Allophycocyanin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: S100A4 Antibody (S100A4/1481) [NBP2-53178] - An intracellular stain was performed on A549 cells with S100A4 Antibody (S100A4/1481) NBP2-54580AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): S100A4 Antibody (S100A4/1481) [NBP2-53178] - An intracellular stain was performed on HeLa cells with S100A4 Antibody (S100A4/1481) NBP2-54580PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Flow Cytometric Analysis of T98G cells using S100A4 Antibody (S100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: S100A4 Antibody (S100A4/1481) [NBP2-53178] - Flow Cytometric Analysis of A549 cells using S100A4 Antibody (S100A4/1481) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).