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LearnH00006275-M01
antibody from Novus Biologicals
Targeting: S100A4
18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00006275-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006275-M01, RRID:AB_547928
- Product name
- Mouse Monoclonal S100A4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. S100A4 - S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references BMP-dependent, injury-induced stem cell niche as a mechanism of heterotopic ossification.
PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma.
Gli1-labeled adult mesenchymal stem/progenitor cells and hedgehog signaling contribute to endochondral heterotopic ossification.
Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer.
Hypoxia-Induced Changes in the Fibroblast Secretome, Exosome, and Whole-Cell Proteome Using Cultured, Cardiac-Derived Cells Isolated from Neonatal Mice.
The essential autophagy gene ATG7 modulates organ fibrosis via regulation of endothelial-to-mesenchymal transition.
Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
MiR-21/Smad 7 signaling determines TGF-β1-induced CAF formation.
Glast-expressing progenitor cells contribute to heterotopic ossification.
Androgen receptor expression in satellite cells of the neonatal levator ani of the rat.
Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.
Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells.
Potential role for S100A4 in the disruption of the blood-brain barrier in collagen-induced arthritic mice, an animal model of rheumatoid arthritis.
Conversion of vascular endothelial cells into multipotent stem-like cells
Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts.
CTGF enhances the motility of breast cancer cells via an integrin-alphavbeta3-ERK1/2-dependent S100A4-upregulated pathway.
Kan C, Ding N, Yang J, Tan Z, McGuire TL, Lu H, Zhang K, Berger DMP, Kessler JA, Kan L
Stem cell research & therapy 2019 Jan 11;10(1):14
Stem cell research & therapy 2019 Jan 11;10(1):14
PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma.
Liu T, Ma W, Xu H, Huang M, Zhang D, He Z, Zhang L, Brem S, O'Rourke DM, Gong Y, Mou Y, Zhang Z, Fan Y
Nature communications 2018 Aug 27;9(1):3439
Nature communications 2018 Aug 27;9(1):3439
Gli1-labeled adult mesenchymal stem/progenitor cells and hedgehog signaling contribute to endochondral heterotopic ossification.
Kan C, Chen L, Hu Y, Ding N, Li Y, McGuire TL, Lu H, Kessler JA, Kan L
Bone 2018 Apr;109:71-79
Bone 2018 Apr;109:71-79
Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer.
Lin SC, Lee YC, Yu G, Cheng CJ, Zhou X, Chu K, Murshed M, Le NT, Baseler L, Abe JI, Fujiwara K, deCrombrugghe B, Logothetis CJ, Gallick GE, Yu-Lee LY, Maity SN, Lin SH
Developmental cell 2017 Jun 5;41(5):467-480.e3
Developmental cell 2017 Jun 5;41(5):467-480.e3
Hypoxia-Induced Changes in the Fibroblast Secretome, Exosome, and Whole-Cell Proteome Using Cultured, Cardiac-Derived Cells Isolated from Neonatal Mice.
Cosme J, Guo H, Hadipour-Lakmehsari S, Emili A, Gramolini AO
Journal of proteome research 2017 Aug 4;16(8):2836-2847
Journal of proteome research 2017 Aug 4;16(8):2836-2847
The essential autophagy gene ATG7 modulates organ fibrosis via regulation of endothelial-to-mesenchymal transition.
Singh KK, Lovren F, Pan Y, Quan A, Ramadan A, Matkar PN, Ehsan M, Sandhu P, Mantella LE, Gupta N, Teoh H, Parotto M, Tabuchi A, Kuebler WM, Al-Omran M, Finkel T, Verma S
The Journal of biological chemistry 2015 Jan 30;290(5):2547-59
The Journal of biological chemistry 2015 Jan 30;290(5):2547-59
Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
Volonté A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):523-32
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):523-32
MiR-21/Smad 7 signaling determines TGF-β1-induced CAF formation.
Li Q, Zhang D, Wang Y, Sun P, Hou X, Larner J, Xiong W, Mi J
Scientific reports 2013;3:2038
Scientific reports 2013;3:2038
Glast-expressing progenitor cells contribute to heterotopic ossification.
Kan L, Peng CY, McGuire TL, Kessler JA
Bone 2013 Mar;53(1):194-203
Bone 2013 Mar;53(1):194-203
Androgen receptor expression in satellite cells of the neonatal levator ani of the rat.
Swift-Gallant A, Monks DA
Developmental neurobiology 2013 Jun;73(6):448-54
Developmental neurobiology 2013 Jun;73(6):448-54
Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.
Birbrair A, Zhang T, Wang ZM, Messi ML, Mintz A, Delbono O
American journal of physiology. Cell physiology 2013 Dec 1;305(11):C1098-113
American journal of physiology. Cell physiology 2013 Dec 1;305(11):C1098-113
Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells.
Chen Y, Huang K, Nakatsu MN, Xue Z, Deng SX, Fan G
Human molecular genetics 2013 Apr 1;22(7):1271-9
Human molecular genetics 2013 Apr 1;22(7):1271-9
Potential role for S100A4 in the disruption of the blood-brain barrier in collagen-induced arthritic mice, an animal model of rheumatoid arthritis.
Nishioku T, Furusho K, Tomita A, Ohishi H, Dohgu S, Shuto H, Yamauchi A, Kataoka Y
Neuroscience 2011 Aug 25;189:286-92
Neuroscience 2011 Aug 25;189:286-92
Conversion of vascular endothelial cells into multipotent stem-like cells
Damian Medici, Eileen M Shore, Vitali Y Lounev, Frederick S Kaplan, Raghu Kalluri, Bjorn R Olsen
Nature Medicine 2010 Nov;16(12):1400-1406
Nature Medicine 2010 Nov;16(12):1400-1406
Discovery of endothelial to mesenchymal transition as a source for carcinoma-associated fibroblasts.
Zeisberg EM, Potenta S, Xie L, Zeisberg M, Kalluri R
Cancer research 2007 Nov 1;67(21):10123-8
Cancer research 2007 Nov 1;67(21):10123-8
CTGF enhances the motility of breast cancer cells via an integrin-alphavbeta3-ERK1/2-dependent S100A4-upregulated pathway.
Chen PS, Wang MY, Wu SN, Su JL, Hong CC, Chuang SE, Chen MW, Hua KT, Wu YL, Cha ST, Babu MS, Chen CN, Lee PH, Chang KJ, Kuo ML
Journal of cell science 2007 Jun 15;120(Pt 12):2053-65
Journal of cell science 2007 Jun 15;120(Pt 12):2053-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 expression in NIH/3T3 ( Cat # L018V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody (M01), clone 1F12-1G7.Lane 1: S100A4 transfected lysate(11.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 monoclonal antibody (M01), clone 1F12-1G7. Analysis of S100A4 expression in Raw 264.7.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: S100A4 Antibody (1F12-1G7) [H00006275-M01] - S100A4 monoclonal antibody (M01), clone 1F12-1G7 Analysis of S100A4 expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Detection limit for recombinant GST tagged S100A4 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: S100A4 Antibody (1F12-1G7) [H00006275-M01] - Analysis of monoclonal antibody to S100A4 on HeLa cell. Antibody concentration 15 ug/ml.
