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LearnNB120-16518
antibody from Novus Biologicals
Targeting: PROM1
AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4
Western blot
ELISA Immunocytochemistry Immunohistochemistry Flow cytometry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NB120-16518 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-16518, RRID:AB_2172880
- Product name
- Rabbit Polyclonal CD133 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. CD133 - Hematopoietic Stem Cell Marker
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1α and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma Rat Model.
Major Improvement in Wound Healing Through Pharmacologic Mobilization of Stem Cells in Severely Diabetic Rats.
Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells.
Renovascular disease induces mitochondrial damage in swine scattered tubular cells.
Interactions with Muscle Cells Boost Fusion, Stemness, and Drug Resistance of Prostate Cancer Cells.
Reversing effect of Lupeol on vasculogenic mimicry in murine melanoma progression.
Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering.
Spontaneous formation of tumor spheroid on a hydrophilic filter paper for cancer stem cell enrichment.
Cell-surface vimentin-positive macrophage-like circulating tumor cells as a novel biomarker of metastatic gastrointestinal stromal tumors.
Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth.
Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles.
GLI1 promotes cancer stemness through intracellular signaling pathway PI3K/Akt/NFκB in colorectal adenocarcinoma.
Characterization of stem cell and cancer stem cell populations in ovary and ovarian tumors.
JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Date Palm (Phoenix dactylifera) Fruits as a Potential Cardioprotective Agent: The Role of Circulating Progenitor Cells.
A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).
A novel method to limit breast cancer stem cells in states of quiescence, proliferation or differentiation: Use of gel stress in combination with stem cell growth factors.
Membranous CD24 drives the epithelial phenotype of pancreatic cancer.
CD133 as a biomarker for putative cancer stem cells in solid tumours: limitations, problems and challenges.
Implanted adipose-derived stem cells attenuate small-for-size liver graft injury by secretion of VEGF in rats.
Prognostic role of CD133 expression in colorectal cancer: a meta-analysis.
Liu J, Dong X, Li Z, Wang G, Zhou Y, Liu C, Wang K, Li L, Liu J
Evidence-based complementary and alternative medicine : eCAM 2020;2020:2305017
Evidence-based complementary and alternative medicine : eCAM 2020;2020:2305017
Major Improvement in Wound Healing Through Pharmacologic Mobilization of Stem Cells in Severely Diabetic Rats.
Qi L, Ahmadi AR, Huang J, Chen M, Pan B, Kuwabara H, Iwasaki K, Wang W, Wesson R, Cameron AM, Cui S, Burdick J, Sun Z
Diabetes 2020 Apr;69(4):699-712
Diabetes 2020 Apr;69(4):699-712
Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells.
Aghajani Nargesi A, Zhu XY, Liu Y, Tang H, Jordan KL, Lerman LO, Eirin A
International journal of molecular sciences 2019 Oct 12;20(20)
International journal of molecular sciences 2019 Oct 12;20(20)
Renovascular disease induces mitochondrial damage in swine scattered tubular cells.
Nargesi AA, Zhu XY, Conley SM, Woollard JR, Saadiq IM, Lerman LO, Eirin A
American journal of physiology. Renal physiology 2019 Nov 1;317(5):F1142-F1153
American journal of physiology. Renal physiology 2019 Nov 1;317(5):F1142-F1153
Interactions with Muscle Cells Boost Fusion, Stemness, and Drug Resistance of Prostate Cancer Cells.
Uygur B, Leikina E, Melikov K, Villasmil R, Verma SK, Vary CPH, Chernomordik LV
Molecular cancer research : MCR 2019 Mar;17(3):806-820
Molecular cancer research : MCR 2019 Mar;17(3):806-820
Reversing effect of Lupeol on vasculogenic mimicry in murine melanoma progression.
Bhattacharyya S, Mitra D, Ray S, Biswas N, Banerjee S, Majumder B, Mustafi SM, Murmu N
Microvascular research 2019 Jan;121:52-62
Microvascular research 2019 Jan;121:52-62
Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering.
Zhao F, Zhou L, Liu J, Xu Z, Ping W, Li H, Xu L, Xu Z, Zhou C, Wang M, Jia R
Journal of tissue engineering 2019 Jan-Dec;10:2041731419891256
Journal of tissue engineering 2019 Jan-Dec;10:2041731419891256
Spontaneous formation of tumor spheroid on a hydrophilic filter paper for cancer stem cell enrichment.
Fu JJ, Zhou Y, Shi XX, Kang YJ, Lu ZS, Li Y, Li CM, Yu L
Colloids and surfaces. B, Biointerfaces 2019 Feb 1;174:426-434
Colloids and surfaces. B, Biointerfaces 2019 Feb 1;174:426-434
Cell-surface vimentin-positive macrophage-like circulating tumor cells as a novel biomarker of metastatic gastrointestinal stromal tumors.
Li H, Meng QH, Noh H, Somaiah N, Torres KE, Xia X, Batth IS, Joseph CP, Liu M, Wang R, Li S
Oncoimmunology 2018;7(5):e1420450
Oncoimmunology 2018;7(5):e1420450
Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth.
Bak DH, Choi MJ, Kim SR, Lee BC, Kim JM, Jeon ES, Oh W, Lim ES, Park BC, Kim MJ, Na J, Kim BJ
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2018 Sep;22(5):555-566
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2018 Sep;22(5):555-566
Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles.
Zou X, Kwon SH, Jiang K, Ferguson CM, Puranik AS, Zhu X, Lerman LO
Scientific reports 2018 Jan 19;8(1):1263
Scientific reports 2018 Jan 19;8(1):1263
GLI1 promotes cancer stemness through intracellular signaling pathway PI3K/Akt/NFκB in colorectal adenocarcinoma.
Yang Z, Zhang C, Qi W, Cui Y, Xuan Y
Experimental cell research 2018 Dec 15;373(1-2):145-154
Experimental cell research 2018 Dec 15;373(1-2):145-154
Characterization of stem cell and cancer stem cell populations in ovary and ovarian tumors.
Parte SC, Batra SK, Kakar SS
Journal of ovarian research 2018 Aug 18;11(1):69
Journal of ovarian research 2018 Aug 18;11(1):69
JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Kim MS, Cho HI, Yoon HJ, Ahn YH, Park EJ, Jin YH, Jang YK
Scientific reports 2018 Apr 26;8(1):6611
Scientific reports 2018 Apr 26;8(1):6611
Date Palm (Phoenix dactylifera) Fruits as a Potential Cardioprotective Agent: The Role of Circulating Progenitor Cells.
Alhaider IA, Mohamed ME, Ahmed KKM, Kumar AHS
Frontiers in pharmacology 2017;8:592
Frontiers in pharmacology 2017;8:592
A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).
Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M
American journal of cancer research 2016;6(12):2799-2815
American journal of cancer research 2016;6(12):2799-2815
A novel method to limit breast cancer stem cells in states of quiescence, proliferation or differentiation: Use of gel stress in combination with stem cell growth factors.
Wang J, Liu X, Jiang Z, Li L, Cui Z, Gao Y, Kong D, Liu X
Oncology letters 2016 Aug;12(2):1355-1360
Oncology letters 2016 Aug;12(2):1355-1360
Membranous CD24 drives the epithelial phenotype of pancreatic cancer.
Lubeseder-Martellato C, Hidalgo-Sastre A, Hartmann C, Alexandrow K, Kamyabi-Moghaddam Z, Sipos B, Wirth M, Neff F, Reichert M, Heid I, Schneider G, Braren R, Schmid RM, Siveke JT
Oncotarget 2016 Aug 2;7(31):49156-49168
Oncotarget 2016 Aug 2;7(31):49156-49168
CD133 as a biomarker for putative cancer stem cells in solid tumours: limitations, problems and challenges.
Grosse-Gehling P, Fargeas CA, Dittfeld C, Garbe Y, Alison MR, Corbeil D, Kunz-Schughart LA
The Journal of pathology 2013 Feb;229(3):355-78
The Journal of pathology 2013 Feb;229(3):355-78
Implanted adipose-derived stem cells attenuate small-for-size liver graft injury by secretion of VEGF in rats.
Ma T, Liu H, Chen W, Xia X, Bai X, Liang L, Zhang Y, Liang T
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Mar;12(3):620-9
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Mar;12(3):620-9
Prognostic role of CD133 expression in colorectal cancer: a meta-analysis.
Wang K, Xu J, Zhang J, Huang J
BMC cancer 2012 Dec 5;12:573
BMC cancer 2012 Dec 5;12:573
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD133 Antibody [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD133 Antibody [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD133 Antibody [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: CD133 Antibody [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
