14112-1-AP

antibody from Invitrogen Antibodies

Targeting: RNF8 KIAA0646

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14112-1-AP

Provider

Invitrogen Antibodies

Product name

RNF8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ETIYPCLSP KNDQMIEKNK ELRTKRKFSL DELAGPGAEG PSNLKSKINK VSCESGQPVK SQGKGEVAST PSDNLDPKLT ALEPSKTTGA PIYPGFPKVT EVHHEQKASN SSASQRSLQM FKVTMSRILR LKIQMQEKHE AVMNVKKQTQ KGNSKKVVQM EQELQDLQSQ LCAEQAQQQA RVEQLEKTFQ EEEQHLQGLE IAQGEKDLKQ QLAQALQEHW ALMEELNRSK KDFEAIIQAK NKELEQTKEE KEKMQAQKEE VLSHMNDVLE NELQCIICSE YFIEAVTLNC AHSFCSYCIN EWMKRKIECP ICRKDIKSKT YSLVLDNCIN KMVNNLSSEV KERRIVLIRE RKAKRLF (130-485 aa encoded by BC007517 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP ( RNF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 14112-1-AP (RNF8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14112-1-AP ( RNF8 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RNF8 (IP:14112-1-AP, 4ug; Detection:14112-1-AP 1:600) with HeLa cells lysate 3200ug.