- GTX111401 - GeneTex CAMK2D antibody

Submitted references CaMKII Potentiates Store-Operated Ca2+ Entry Through Enhancing STIM1 Aggregation and Interaction with Orai1.

Li S, Xue J, Sun Z, Liu T, Zhang L, Wang L, You H, Fan Z, Zheng Y, Luo D
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(3):1042-1054

Gallic acid attenuates calcium calmodulin-dependent kinase II-induced apoptosis in spontaneously hypertensive rats.

Jin L, Piao ZH, Liu CP, Sun S, Liu B, Kim GR, Choi SY, Ryu Y, Kee HJ, Jeong MH
Journal of cellular and molecular medicine 2018 Mar;22(3):1517-1526

Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca(2+) Affinity of the Multi-C(2)-Domain Protein Otoferlin.

Meese S, Cepeda AP, Gahlen F, Adams CM, Ficner R, Ricci AJ, Heller S, Reisinger E, Herget M
Frontiers in synaptic neuroscience 2017;9:13

CaMKII induces permeability transition through Drp1 phosphorylation during chronic β-AR stimulation.

Xu S, Wang P, Zhang H, Gong G, Gutierrez Cortes N, Zhu W, Yoon Y, Tian R, Wang W
Nature communications 2016 Oct 14;7:13189

Post-translational dual regulation of cytochrome P450 aromatase at the catalytic and protein levels by phosphorylation/dephosphorylation.

Hayashi T, Harada N
The FEBS journal 2014 Nov;281(21):4830-40

Mechanisms underlying a decrease in KCl-induced contraction after long-term serum-free organ culture of rat isolated mesenteric artery.

Morita T, Okada M, Yamawaki H
The Journal of veterinary medical science 2014 Jul;76(7):963-9

FGF-23 dysregulates calcium homeostasis and electrophysiological properties in HL-1 atrial cells.

Kao YH, Chen YC, Lin YK, Shiu RJ, Chao TF, Chen SA, Chen YJ
European journal of clinical investigation 2014 Aug;44(8):795-801

Histone deacetylase inhibition improved cardiac functions with direct antifibrotic activity in heart failure.

Kao YH, Liou JP, Chung CC, Lien GS, Kuo CC, Chen SA, Chen YJ
International journal of cardiology 2013 Oct 9;168(4):4178-83

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A431 (GTX27909) B: H1299 10% SDS PAGE GTX111401 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX111401 diluted at 1:10000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMKII delta antibody detects CaMKII delta protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract10 % SDS-PAGECaMKII delta antibody (GTX111401) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMK2D antibody detects CaMK2D protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract10 % SDS-PAGECaMK2D antibody (GTX111401) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMK2D antibody detects CaMK2D protein by western blot analysis.A. 30 ?g A549 whole cell lysate/extract B. 30 ?g H1299 whole cell lysate/extract C. 30 ?g HCT116 whole cell lysate/extract10% SDS-PAGECaMK2D antibody (GTX111401) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMK2D antibody detects CaMK2D protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 10 % SDS-PAGE, and the membrane was blotted with CaMK2D antibody (GTX111401) diluted by 1:10000.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMKII delta antibody detects CaMKII delta protein at cytoplasm by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CaMKII delta protein stained by CaMKII delta antibody (GTX111401) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of CaMKII delta protein from HepG2 whole cell extracts using 5 £gg of CaMKII delta antibody (GTX111401).Western blot analysis was performed using CaMKII delta antibody (GTX111401).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMKII delta antibody detects CaMKII delta protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CaMKII delta antibody (GTX111401) diluted at 1:400.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CaMKII delta antibody detects CaMKII delta protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. CaMKII delta antibody (GTX111401) diluted at 1:400.

GTX111401

Antibody data