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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [4]
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- Product number
- PA5-110977 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NRELRDCFRA QFPCCQSPRT TQATHPCDLK GIGL
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.30 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references GPR27 Regulates Hepatocellular Carcinoma Progression via MAPK/ERK Pathway.
Wang H, Du D, Huang J, Wang S, He X, Yuan S, Xiao J
Cancer management and research 2022;14:1165-1177
Cancer management and research 2022;14:1165-1177
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR27 in human cell line NTERA-2 using GPR27 Polyclonal Antibody (Product # PA5-110977).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR27 in human cell line NTERA-2 using GPR27 Polyclonal Antibody (Product # PA5-110977).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of GPR27 in human cell line U-2 OS using GPR27 Polyclonal Antibody (Product # PA5-110977) shows localization to nucleoplasm, plasma membrane and actin filaments.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of GPR27 inhibits HCC cells proliferation in vitro. ( A ) The mRNA and protein expression of GPR27 were examined in HCC cell lines. ( B and C ) GPR27 knockdown efficiency of siRNAs was determined by qRT-PCR and Western blot analysis. ( D ) Colony formation assays were performed to determine the cell clonogenicity of SMMC-7721 and SK-Hep-1 cells treated with control or GPR27 siRNAs. ( E ) The effects of GPR27 on cell proliferation of SMMC-7721 and SK-Hep-1 cells were detected by measuring the intracellular ATP content treated with control or GPR27 siRNAs. ( F ) Real Time Cellular Analysis (RTCA) assays were performed to evaluate the effect of GPR27 on HCC cell proliferation treated with control or GPR27 siRNAs. Data are represented as the mean +- SD of three independent experiments. The p-values < 0.05 were considered statistically significant for all tests.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Overexpression of GPR27 promotes HCC cells proliferation in vitro. ( A ) GPR27 overexpression efficiency was determined by qRT-PCR and Western blot analysis after lentiviral infection in SMMC-7721 and SK-Hep-1 cells. ( B ) Colony formation assays were performed to determine the cell clonogenicity of GPR27-OE SMMC-7721 and SK-Hep-1 cells. ( C ) Growth curve analysis was conducted to examine the cell proliferation in GPR27-OE SMMC-7721 and SK-Hep-1 cells. Data are represented as the mean +- SD of three independent experiments. The p-values < 0.05 were considered statistically significant for all tests.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of GPR27 induces S phase arrest in HCC cells. ( A ) Cell cycle distribution was measured by flow cytometry using PI staining in SMMC-7721 and SK-Hep-1 cells treated with control or GPR27 siRNAs. ( B ) The cell cycle related proteins were analyzed through Western blot analysis after GPR27 knockdown. Data are represented as the mean +- SD of three independent experiments. The p-values < 0.05 were considered statistically significant for all tests (*P < 0.05, **P < 0.01, ***P < 0.001 vs SiNC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 GPR27 knockdown inhibits HCC growth in vivo. ( A - E ). SMMC-7721 cells (2 x 10 6 cells per mouse) were injected into the axilla of 6-week-old male BALB/c nude mice (n=6). When tumor volume reaches at 100 mm 3 , the control siRNA or GPR27 siRNA (10 mug per tumor) was injected into tumors every other day. The size ( D ) of the tumors and body weight ( C ) of mice were measured every 2 or 3 days. Nude mice were sacrificed on day 25 after inoculation, xenograft tumors were harvested. Tumor growth curves ( D ), photographs of mice ( A ) and harvested tumors ( B ), and tumor weight ( E ) are shown. ( F ) Representative images of H&E-stained sections of tumors and Ki67 positive cells ( F ) are shown. Scale bar: 50mum. ( G and H ) GPR27 knockdown efficiency in vivo was validated by qRT-PCR ( G ) and Western blot analysis ( H ). And the protein expression levels of c-Raf, p-c-Raf, ERK1/2, p-ERK1/2, MEK and p-MEK in tumor tissues were determined by Western blot. The p-values < 0.05 were considered statistically significant.
