Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 14-5936-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VEGF Receptor 1 (Flt1) Monoclonal Antibody (7A6), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 7A6 monoclonal antibody recognizes human VEGFR1 (vascular endothelial growth factor receptor 1, fms-like tyrosine kinase 1, FLT1). It is directed against the extracellular domain and can recognize both membrane-bound and soluble forms of VEGFR1, with approximate molecular weights of 180 kDa and 90 kDa, respectively. Mice lacking expression of VEGFR1 have disorganized vasculature and die in utero, however angiogenesis does not require the tyrosine kinase activity of VEGFR1 as expression of a membrane-bound form lacking the tyrosine kinase domain allows normal development. Although fertile and viable, mice lacking the tyrosine kinase domain of VEGFR1 do have a defect in the migration of macrophages. The soluble form of VEGFR1 can be detected in the serum of normal patients, and elevated levels of soluble VEGFR1 in pregnant women is associated with preeclampsia. Several tumor cell lines have been reported to express membrane-bound VEGFR1, including malignancies of hematopoietic origin. Applications Reported: This 7A6 antibody has been reported for use in immunoblotting (WB). Applications Tested: This 7A6 antibody has been tested by immunoblot analysis of HUVEC. It can be used at 1-5 µg/mL. This antibody only recognizes non-reduced epitopes. It is highly recommended to include protease inhibitors in the lysing buffer and to not boil lysates prior to separation by non-reducing SDS-PAGE. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 7A6
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references The protein tyrosine kinase inhibitor SU5614 inhibits VEGF-induced endothelial cell sprouting and induces growth arrest and apoptosis by inhibition of c-kit in AML cells.
Soluble VEGFR-1 secreted by endothelial cells and monocytes is present in human serum and plasma from healthy donors.
Structure and dual function of vascular endothelial growth factor receptor-1 (Flt-1).
Expression of vascular endothelial growth factor and its receptors in hematopoietic malignancies.
Spiekermann K, Faber F, Voswinckel R, Hiddemann W
Experimental hematology 2002 Jul;30(7):767-73
Experimental hematology 2002 Jul;30(7):767-73
Soluble VEGFR-1 secreted by endothelial cells and monocytes is present in human serum and plasma from healthy donors.
Barleon B, Reusch P, Totzke F, Herzog C, Keck C, Martiny-Baron G, Marmé D
Angiogenesis 2001;4(2):143-54
Angiogenesis 2001;4(2):143-54
Structure and dual function of vascular endothelial growth factor receptor-1 (Flt-1).
Shibuya M
The international journal of biochemistry & cell biology 2001 Apr;33(4):409-20
The international journal of biochemistry & cell biology 2001 Apr;33(4):409-20
Expression of vascular endothelial growth factor and its receptors in hematopoietic malignancies.
Bellamy WT, Richter L, Frutiger Y, Grogan TM
Cancer research 1999 Feb 1;59(3):728-33
Cancer research 1999 Feb 1;59(3):728-33
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunoblot analysis of non-boiled NP-40 lysates from Human Umbilical Vein Endothelial Cells (HUVEC) run on SDS-PAGE under non-reducing conditions. The membrane was probed with 2 µg/mL of Anti-Human VEGF Receptor 1 (Flt1) Purified followed by Anti-Rat IgG HRP.