LS-C313208

antibody from LSBio

Targeting: CCN4 WISP-1, WISP1, WISP1-OT1, WISP1-UT1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C313208

Provider

LSBio

Product name

CCN4 / WISP1 Antibody (aa354-367) LS-C313208

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Horse, Rabbit, Simian

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CCN4 / WISP1 antibody. ICC: MCF-7 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CCN4 / WISP1 antibody. ICC: MCF-7 Cell.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CCN4 / WISP1 antibody. IHC(P): Rat Kidney Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CCN4 / WISP1 antibody. IHC(P): Rat Pancreas Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

CCN4 / WISP1 antibody. IHC(P): Human Placenta Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC analysis of WISP1 using anti-WISP1 antibody. WISP1 was detected in frozen section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-WISP1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CCN4 / WISP1 antibody. IHC(P): Rat Kidney Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CCN4 / WISP1 antibody. IHC(P): Rat Pancreas Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

CCN4 / WISP1 antibody. IHC(P): Human Placenta Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC analysis of WISP1 using anti-WISP1 antibody. WISP1 was detected in frozen section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-WISP1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.