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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-WISP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-29150 targets WISP1 in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-29150 immunogen is synthetic peptide corresponding to a region within amino acids 135 and 230 of Human WISP1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references WISP-1 overexpression upregulates cell proliferation in human salivary gland carcinomas via regulating MMP-2 expression.
Li FJ, Wang XJ, Zhou XL
OncoTargets and therapy 2016;9:6539-6548
OncoTargets and therapy 2016;9:6539-6548
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WISP1 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a WISP1 polyclonal antibody (Product # PA5-29150) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of WISP1 in paraffin-embedded HT-29 xenograft using a WISP1 polyclonal antibody (Product # PA5-29150) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WISP1 in methanol-fixed A431 cells using a WISP1 polyclonal antibody (Product # PA5-29150) at a 1:200 dilution.
