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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-36985 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-36985, RRID:AB_2270232
- Product name
- Goat Polyclonal sFRP-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
internal region- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R
Genes & development 2007 Dec 1;21(23):3110-22
Genes & development 2007 Dec 1;21(23):3110-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: sFRP-4 Antibody [NBP1-36985] - Western blot analysis of SFRP4 in Human Uterus lysate (35ug protein in RIPA buffer) using NBP1-36985 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985] - (3.8ug/ml) staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP1-36985] - Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland.
