Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG90005 - Provider product page
- Provider
- Arigo
- Product name
- anti-GLP-1 Receptor antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around the center region of Human GLP-1 Receptor.
- Description
- Affinity purification with immunogen.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: (A) SKNSH and (B)HUVEC whole cell lysates stained with ARG90005 anti-GLP-1 Receptor antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: SKNSH cells stained with ARG90005 anti-GLP-1 Receptor antibody (red). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at RT. Cells were stained with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: Formalin-fixed, paraffin embedded Human brain section stained with ARG90005 anti-GLP-1 Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0), then incubated with the antibody at RT and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
