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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
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- Product number
- PA5-120841 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HeLa, 293T, Jurkat, LO2, Mouse brain. Immunogen sequence: PKTINQIRQD AVKDLGVFIP APMAQGMRSD FFLEGPFMPP RMKMDRDPLG GLADMFGQMP GSGIGTGPGV IQDRFSPTMG RHRSNQLFNG HGGHIMPPTQ SQFGEMGGKF MKSQGLSQLY HNQSQGLLSQ LQGQSKDMPP RFSKKGQLNA DEISLRPAQS FLMNKNQVPK L
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.26 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DAP5 in HeLa cells using a DAP5 Polyclonal antibody (Product # PA5-120841) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DAP5 in various cell lines using a DAP5 Polyclonal antibody (Product # PA5-120841) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of DAP5 in C6 cells using a DAP5 Polyclonal antibody (Product # PA5-120841). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of DAP5 in mouse brain using a Polyclonal antibody (Product # PA5-120841) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
