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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB42941 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-22 R alpha 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-22 R alpha 1 in direct ELISAs. In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL-22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha , recombinant mouse (rm) IL-20 R alpha , rhIL-22BP, or rmIL-22BP is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q80XZ4- Isotype
- IgG
- Antibody clone number
- 496514
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae.
Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy.
Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection.
A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice.
Endogenous IL-22 plays a dual role in arthritis: regulation of established arthritis via IFN-γ responses.
IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
IL-23-mediated epidermal hyperplasia is dependent on IL-6.
CCL20, γδ T cells, and IL-22 in corneal epithelial healing.
Madouri F, Barada O, Kervoaze G, Trottein F, Pichavant M, Gosset P
EBioMedicine 2018 Nov;37:417-427
EBioMedicine 2018 Nov;37:417-427
Interleukin-20 targets podocytes and is upregulated in experimental murine diabetic nephropathy.
Hsu YH, Li HH, Sung JM, Chen WY, Hou YC, Weng YH, Lai WT, Wu CH, Chang MS
Experimental & molecular medicine 2017 Mar 31;49(3):e310
Experimental & molecular medicine 2017 Mar 31;49(3):e310
Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection.
Treerat P, Prince O, Cruz-Lagunas A, Muñoz-Torrico M, Salazar-Lezama MA, Selman M, Fallert-Junecko B, Reinhardt TA, Alcorn JF, Kaushal D, Zuñiga J, Rangel-Moreno J, Kolls JK, Khader SA
Mucosal immunology 2017 Jul;10(4):1069-1081
Mucosal immunology 2017 Jul;10(4):1069-1081
A TLR9 agonist promotes IL-22-dependent pancreatic islet allograft survival in type 1 diabetic mice.
Tripathi D, Venkatasubramanian S, Cheekatla SS, Paidipally P, Welch E, Tvinnereim AR, Vankayalapati R
Nature communications 2016 Dec 16;7:13896
Nature communications 2016 Dec 16;7:13896
Endogenous IL-22 plays a dual role in arthritis: regulation of established arthritis via IFN-γ responses.
Justa S, Zhou X, Sarkar S
PloS one 2014;9(3):e93279
PloS one 2014;9(3):e93279
IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection.
Kumar P, Thakar MS, Ouyang W, Malarkannan S
Mucosal immunology 2013 Jan;6(1):69-82
Mucosal immunology 2013 Jan;6(1):69-82
IL-23-mediated epidermal hyperplasia is dependent on IL-6.
Lindroos J, Svensson L, Norsgaard H, Lovato P, Moller K, Hagedorn PH, Olsen GM, Labuda T
The Journal of investigative dermatology 2011 May;131(5):1110-8
The Journal of investigative dermatology 2011 May;131(5):1110-8
CCL20, γδ T cells, and IL-22 in corneal epithelial healing.
Li Z, Burns AR, Miller SB, Smith CW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2659-68
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2659-68
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-22 R alpha 1 in Hepa 1-6 Mouse Cell Line. IL-22 R alpha 1 was detected in immersion fixed Hepa 1-6 mouse hepatoma cell line using Rat Anti-Mouse IL-22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-22 R alpha 1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Rat Anti-Mouse IL-22 R alpha 1 Monoclonal Antibody (Catalog # MAB42941, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
