GTX101766

antibody from GeneTex

Targeting: CD81 TAPA-1, TAPA1, TSPAN28

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

GTX101766

Provider

GeneTex

Proper citation

GeneTex Cat#GTX101766, RRID:AB_10618892

Product name

CD81 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer.

Hwang WL, Lan HY, Cheng WC, Huang SC, Yang MH
Journal of hematology & oncology 2019 Jan 25;12(1):10

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Extracellular Vesicles Secreted by Astroglial Cells Transport Apolipoprotein D to Neurons and Mediate Neuronal Survival Upon Oxidative Stress.

Pascua-Maestro R, González E, Lillo C, Ganfornina MD, Falcón-Pérez JM, Sanchez D
Frontiers in cellular neuroscience 2018;12:526

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EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.

Lee HY, Chen CK, Ho CM, Lee SS, Chang CY, Chen KJ, Jou YS
Oncotarget 2018 Mar 2;9(17):13193-13205

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Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes.

Xue Y, Mars WM, Bowen W, Singhi AD, Stoops J, Michalopoulos GK
The American journal of pathology 2018 Jun;188(6):1469-1477

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Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes.

Lin MC, Chen SY, He PL, Luo WT, Li HJ
Journal of visualized experiments : JoVE 2017 Jun 3;(124)

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Human immunodeficiency virus type 1 Vpr increases hepatitis C virus RNA replication in cell culture.

Deng A, Chen C, Ishizaka Y, Chen X, Sun B, Yang R
Virus research 2014 May 12;184:93-102

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: K562 12% SDS PAGE GTX101766 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE GTX101766 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD81 antibody detects CD81 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD81 protein stained by CD81 antibody (GTX101766) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using CD81(GTX101766) antibody at 1:250 dilution.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis. Sample: K562 cell. Black: Unlabelled sample was used as a control. Red: CD81 antibody (GTX101766) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Validation comment

FACS

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD81 antibody (GTX101766) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD81 antibody (GTX101766) dilution: 1:50.The Rabbit IgG antibody (DyLight488) (GTX213110-04) was used to detect the primary antibody.