- NB100-65805 - Novus Biologicals CD81 antibody

Submitted references Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.

Roma-Rodrigues C, Fernandes AR, Baptista PV
International journal of nanomedicine 2019;14:6843-6854

Multiplexed profiling of single-cell extracellular vesicles secretion.

Ji Y, Qi D, Li L, Su H, Li X, Luo Y, Sun B, Zhang F, Lin B, Liu T, Lu Y
Proceedings of the National Academy of Sciences of the United States of America 2019 Mar 26;116(13):5979-5984

Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid.

Otake K, Kamiguchi H, Hirozane Y
BMC medical genomics 2019 Jan 10;12(1):7

Extracellular vesicles secreted by hypoxia pre-challenged mesenchymal stem cells promote non-small cell lung cancer cell growth and mobility as well as macrophage M2 polarization via miR-21-5p delivery.

Ren W, Hou J, Yang C, Wang H, Wu S, Wu Y, Zhao X, Lu C
Journal of experimental & clinical cancer research : CR 2019 Feb 8;38(1):62

Correlation between Cancerous Exosomes and Protein Markers Based on Surface-Enhanced Raman Spectroscopy (SERS) and Principal Component Analysis (PCA).

Shin H, Jeong H, Park J, Hong S, Choi Y
ACS sensors 2018 Dec 28;3(12):2637-2643

A rapid, automated surface protein profiling of single circulating exosomes in human blood.

Kibria G, Ramos EK, Lee KE, Bedoyan S, Huang S, Samaeekia R, Athman JJ, Harding CV, Lötvall J, Harris L, Thompson CL, Liu H
Scientific reports 2016 Nov 7;6:36502

Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81.

Tian ZF, Shen H, Fu XH, Chen YC, Blum HE, Baumert TF, Zhao XP
World journal of gastroenterology 2009 Jan 14;15(2):240-4

Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81.

Tian ZF, Shen H, Fu XH, Chen YC, Blum HE, Baumert TF, Zhao XP
World journal of gastroenterology 2009 Jan 14;15(2):240-4

Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.

Flint M, Maidens C, Loomis-Price LD, Shotton C, Dubuisson J, Monk P, Higginbottom A, Levy S, McKeating JA
Journal of virology 1999 Aug;73(8):6235-44

Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.

Flint M, Maidens C, Loomis-Price LD, Shotton C, Dubuisson J, Monk P, Higginbottom A, Levy S, McKeating JA
Journal of virology 1999 Aug;73(8):6235-44

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36502), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

NB100-65805

Antibody data