20789-1-AP

antibody from Invitrogen Antibodies

Targeting: FANCI FLJ10719, KIAA1794

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20789-1-AP

Provider

Invitrogen Antibodies

Product name

FANCI Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ATMDQKILS LAAEKTADKL QEFLQTLREG DLTNLLQNQA VKGKVAGALL RAIFKGSPCS EEAGTLRRRK IYTCCIQLVE SGDLQKEIVS EIIGLLMLEA HHFPGPLLVE LANEFISAVR EGSLVNGKSL ELLPIILTAL ATKKENLAYG KGVLSGEECK KQLINTLCSG RWDQQYVIQL TSMFKDVPLT AEEVEFVVEK ALSMFSKMNL QEIPPLVYQL LVLSSKGSRK SVLEGIIAFF SALDKQHNEE QSGDE (1-252 aa encoded by BC004277)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20789-1-AP (FANCI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 20789-1-AP (FANCI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 20789-1-AP (FANCI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using FANCI antibody 20789-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-FANCI (IP:20789-1-AP, 4ug; Detection:20789-1-AP 1:1000) with Jurkat cells lysate 2400ug.