Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX104680 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104680, RRID:AB_1241033
- Product name
- MAD2L1 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references The spindle checkpoint protein MAD1 regulates the expression of E-cadherin and prevents cell migration.
Chen Y, Yeh PC, Huang JC, Yeh CC, Juang YL
Oncology reports 2012 Feb;27(2):487-91
Oncology reports 2012 Feb;27(2):487-91
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g whole cell lysate)A:Hep G2 (GTX27900)B:MOLT4 (GTX27912)C:Raji (GTX27908)12% SDS PAGEGTX104680 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 £gg of MAD2L1 antibody [C2C3], C-term (GTX104680).Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.
