H00004085-M01

antibody from Abnova Corporation

Targeting: MAD2L1 HSMAD2, MAD2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004085-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004085-M01, RRID:AB_425533

Product name

MAD2L1 monoclonal antibody (M01), clone 2E2-1D6

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MAD2L1.

Antigen sequence

MALQLSREQGITLRGSAEIVAEFFSFGINSILYQR
GIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVE
QLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIE
CDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVT
FLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFI
TNSEEVRLRSFTTTIHKVNSMVAYKIPVND


Isotype

IgG

Antibody clone number

2E2-1D6

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

RED, a spindle pole-associated protein, is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint.

Yeh PC, Yeh CC, Chen YC, Juang YL
The Journal of biological chemistry 2012 Apr 6;287(15):11704-16

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MAD2L1 monoclonal antibody (M01), clone 2E2-1D6 Western Blot analysis of MAD2L1 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAD2L1 expression in transfected 293T cell line by MAD2L1 monoclonal antibody (M01), clone 2E2-1D6.Lane 1: MAD2L1 transfected lysate(23.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAD2L1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol