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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1926 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1926, RRID:AB_1679501
- Product name
- MAD2L1 monoclonal antibody, clone 17D10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant.
- Isotype
- IgG
- Antibody clone number
- 17D10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Kinetochore structure and function.
Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells.
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
Chan GK, Liu ST, Yen TJ
Trends in cell biology 2005 Nov;15(11):589-98
Trends in cell biology 2005 Nov;15(11):589-98
Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells.
Sudakin V, Yen TJ
Methods in molecular biology (Clifton, N.J.) 2004;281:199-212
Methods in molecular biology (Clifton, N.J.) 2004;281:199-212
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M
Current biology : CB 2004 Apr 6;14(7):611-7
Current biology : CB 2004 Apr 6;14(7):611-7
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using MAD2L1 monoclonal antibody, clone 17D10 (Cat # MAB1926) shows detection of aband at ~24 KDa (arrowhead) correspondingto MAD2L1 present in a HeLa whole cell lysate (Lane 1).Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE.After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200.The membrane was washed and reacted with a 1 : 5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 minat room temperature (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).IRDye™800 fluorescence image was captured using the Odyssey® InfraredImaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc. Otherdetection systems will yield similar results.
