600-401-461

antibody from Rockland Immunochemicals, Inc.

Targeting: MAD2L1 HSMAD2, MAD2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-461

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-461, RRID:AB_217969

Product name

Anti-MAD2L1 (RABBIT) Antibody - 600-401-461

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kDa corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~ 35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu (611-432-122) for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

Western Blot