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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- NB100-563 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-563, RRID:AB_10001324
- Product name
- Rabbit Polyclonal MAD2L1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment.
Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis.
Effects of spindle poisons in peripheral human lymphocytes by the in vitro cytokinesis-block micronucleus assay.
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation.
p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption.
Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator.
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Bub1 regulates chromosome segregation in a kinetochore-independent manner.
Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis.
The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions.
Retinoblastoma pathway dysregulation causes DNA methyltransferase 1 overexpression in cancer via MAD2-mediated inhibition of the anaphase-promoting complex.
The anaphase-promoting complex coordinates initiation of lens differentiation.
Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells.
Itoh G, Ikeda M, Iemura K, Amin MA, Kuriyama S, Tanaka M, Mizuno N, Osakada H, Haraguchi T, Tanaka K
Scientific reports 2018 Mar 1;8(1):3888
Scientific reports 2018 Mar 1;8(1):3888
Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis.
Ikeda M, Tanaka K
Scientific reports 2017 Aug 18;7(1):8794
Scientific reports 2017 Aug 18;7(1):8794
Effects of spindle poisons in peripheral human lymphocytes by the in vitro cytokinesis-block micronucleus assay.
Guccini S, Lombardi S, Pisani A, Piaggi S, Scarpato R
Mutagenesis 2012 Nov;27(6):749-58
Mutagenesis 2012 Nov;27(6):749-58
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Matson DR, Demirel PB, Stukenberg PT, Burke DJ
Genes & development 2012 Mar 15;26(6):542-7
Genes & development 2012 Mar 15;26(6):542-7
Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation.
McHedlishvili N, Wieser S, Holtackers R, Mouysset J, Belwal M, Amaro AC, Meraldi P
Journal of cell science 2012 Feb 15;125(Pt 4):906-18
Journal of cell science 2012 Feb 15;125(Pt 4):906-18
p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption.
Yun M, Han YH, Yoon SH, Kim HY, Kim BY, Ju YJ, Kang CM, Jang SH, Chung HY, Lee SJ, Cho MH, Yoon G, Park GH, Kim SH, Lee KH
Molecular cancer research : MCR 2009 Mar;7(3):371-82
Molecular cancer research : MCR 2009 Mar;7(3):371-82
Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator.
Lince-Faria M, Maffini S, Orr B, Ding Y, Cláudia Florindo, Sunkel CE, Tavares A, Johansen J, Johansen KM, Maiato H
The Journal of cell biology 2009 Mar 9;184(5):647-57
The Journal of cell biology 2009 Mar 9;184(5):647-57
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A
The Journal of cell biology 2009 Jun 1;185(5):859-74
The Journal of cell biology 2009 Jun 1;185(5):859-74
Bub1 regulates chromosome segregation in a kinetochore-independent manner.
Klebig C, Korinth D, Meraldi P
The Journal of cell biology 2009 Jun 1;185(5):841-58
The Journal of cell biology 2009 Jun 1;185(5):841-58
Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis.
Santamaria A, Neef R, Eberspächer U, Eis K, Husemann M, Mumberg D, Prechtl S, Schulze V, Siemeister G, Wortmann L, Barr FA, Nigg EA
Molecular biology of the cell 2007 Oct;18(10):4024-36
Molecular biology of the cell 2007 Oct;18(10):4024-36
The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions.
Vader G, Cruijsen CW, van Harn T, Vromans MJ, Medema RH, Lens SM
Molecular biology of the cell 2007 Nov;18(11):4553-64
Molecular biology of the cell 2007 Nov;18(11):4553-64
Retinoblastoma pathway dysregulation causes DNA methyltransferase 1 overexpression in cancer via MAD2-mediated inhibition of the anaphase-promoting complex.
Agoston AT, Argani P, De Marzo AM, Hicks JL, Nelson WG
The American journal of pathology 2007 May;170(5):1585-93
The American journal of pathology 2007 May;170(5):1585-93
The anaphase-promoting complex coordinates initiation of lens differentiation.
Wu G, Glickstein S, Liu W, Fujita T, Li W, Yang Q, Duvoisin R, Wan Y
Molecular biology of the cell 2007 Mar;18(3):1018-29
Molecular biology of the cell 2007 Mar;18(3):1018-29
Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells.
Ito S, Mantel CR, Han MK, Basu S, Fukuda S, Cooper S, Broxmeyer HE
Blood 2007 Mar 1;109(5):1923-30
Blood 2007 Mar 1;109(5):1923-30
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2 on HeLa whole cell lysate using NB100-563. MAD2 was also immunoprecipitated using NB100-564. Immunoprecipitated MAD2 was blotted using NB100-564.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
