BS-7717R

antibody from Invitrogen Antibodies

Targeting: CDCA5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-7717R

Provider

Invitrogen Antibodies

Product name

Sororin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti CDCA5/Sororin Polyclonal Antibody, Unconjugated (bs-7717R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band 28 kD. Observed band size: 28 kD.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti CDCA5/Sororin Polyclonal Antibody, Unconjugated (bs-7717R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band 28 kD. Observed band size: 28 kD.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Antigen: bs-7717P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000; TMB staining; Read the data in Microplate Reader by 450nm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti CDCA5/Sororin Polyclonal Antibody, Unconjugated (bs-7717R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.