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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB300-214 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-214, RRID:AB_10002270
- Product name
- Rabbit Polyclonal TCERG1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The transcriptional transactivator Tat selectively regulates viral splicing.
Enzymatic activity is required for the in vivo functions of CARM1.
The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing.
Identification of a co-repressor that inhibits the transcriptional and growth-arrest activities of CCAAT/enhancer-binding protein alpha.
Jablonski JA, Amelio AL, Giacca M, Caputi M
Nucleic acids research 2010 Mar;38(4):1249-60
Nucleic acids research 2010 Mar;38(4):1249-60
Enzymatic activity is required for the in vivo functions of CARM1.
Kim D, Lee J, Cheng D, Li J, Carter C, Richie E, Bedford MT
The Journal of biological chemistry 2010 Jan 8;285(2):1147-52
The Journal of biological chemistry 2010 Jan 8;285(2):1147-52
The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing.
Cheng D, Côté J, Shaaban S, Bedford MT
Molecular cell 2007 Jan 12;25(1):71-83
Molecular cell 2007 Jan 12;25(1):71-83
Identification of a co-repressor that inhibits the transcriptional and growth-arrest activities of CCAAT/enhancer-binding protein alpha.
McFie PJ, Wang GL, Timchenko NA, Wilson HL, Hu X, Roesler WJ
The Journal of biological chemistry 2006 Jun 30;281(26):18069-80
The Journal of biological chemistry 2006 Jun 30;281(26):18069-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCERG1 Antibody [NB300-214] - Detection of Human CA150 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CA150 antibody NB300-214 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCERG1 Antibody [NB300-214] - Nuclear extract (5 ug) from HeLa cells. Antibody used at 0.33 ug/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TCERG1 Antibody [NB300-214] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-CA150 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
