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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF6615 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Perilipin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Perilipin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O60240- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration.
Himburg HA, Termini CM, Schlussel L, Kan J, Li M, Zhao L, Fang T, Sasine JP, Chang VY, Chute JP
Cell stem cell 2018 Sep 6;23(3):370-381.e5
Cell stem cell 2018 Sep 6;23(3):370-381.e5
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Perilipin by Western Blot. Western blot shows lysates of human heart (adipose) tissue. PVDF Membrane was probed with 0.25 µg/mL of Human Perilipin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6615) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Perilipin at approximately 55-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
