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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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- Product number
- NB500-177 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-177, RRID:AB_2086800
- Product name
- Rabbit Polyclonal CTCF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references CTCF binding to the first intron of the major immediate early (MIE) gene of human cytomegalovirus (HCMV) negatively regulates MIE gene expression and HCMV replication.
MartÃnez FP, Cruz R, Lu F, Plasschaert R, Deng Z, Rivera-Molina YA, Bartolomei MS, Lieberman PM, Tang Q
Journal of virology 2014 Jul;88(13):7389-401
Journal of virology 2014 Jul;88(13):7389-401
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CTCF Antibody [NB500-177] - Samples: A) Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CTCF Antibody [NB500-177] - Detection of Mouse CTCF by Western Blot. Samples: Whole cell lysate (50 ug) from CT26.WT, 4T1, Renca, and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CTCF Antibody [NB500-177] - Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 (lot NB500-177-6) used for IP at 6 ug per reaction. CTCF was also immunoprecipitated by a previous lot of this antibody (lot NB500-177-5). For blotting immunoprecipitated CTCF, NB500-177 was used at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CTCF Antibody [NB500-177] - Sample: FFPE sections of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 CTCFfs20 used at a dilution of 1:100 (2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: CTCF Antibody [NB500-177] - Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 20 micrograms of rabbit anti-CTCF antibody NB500-177. The resulting ChIP DNA was quantified using real-time PCR with primers against the IGF2-H19 ICR or a control region in the HoxA cluster.
