Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00007153-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007153-M01, RRID:AB_548767
- Product name
- Mouse Monoclonal TOP2A Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TOP2A - topoisomerase (DNA) II alpha 170kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins.
Poonperm R, Takata H, Hamano T, Matsuda A, Uchiyama S, Hiraoka Y, Fukui K
Scientific reports 2015 Jul 1;5:11916
Scientific reports 2015 Jul 1;5:11916
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOP2A Antibody (1E2) [H00007153-M01] - TOP2A monoclonal antibody (M01), clone 1E2 Analysis of TOP2A expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TOP2A Antibody (1E2) [H00007153-M01] - Detection limit for recombinant GST tagged TOP2A is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TOP2A Antibody (1E2) [H00007153-M01] - Analysis of monoclonal antibody to TOP2A on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP2A Antibody (1E2) [H00007153-M01] - Analysis of monoclonal antibody to TOP2A on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.