24641-1-AP

antibody from Invitrogen Antibodies

Targeting: TOP2A TOP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24641-1-AP

Provider

Invitrogen Antibodies

Product name

TOP2A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QDEQVGLPG KGGKAKGKKT QMAEVLPSPR GQRVIPRITI EMKAEAEKKN KKKIKNENTE GSPQEDGVEL EGLKQRLEKK QKREPGTKTK KQTTLAFKPI KKGKKRNPWS DSESDRSSDE SNFDVPPRET EPRRAATKTK FTMDLDSDED FSDFDEKTDD EDFVPSDASP PKTKTSPKLS NKELKPQKSV VSDLEADDVK GSVPLSSSPP ATHFPDETEI TNPVPKKNVT VKKTAAKSQS STSTTGAKKR AAPKGTKRDP ALNSGVSQKP DPAKTKNRRK RKPSTSDDSD SNFEKIVSKA VTSKKSKGES DDFHMDFDSA VAPRAKSVRA KKPIKYLEES DEDDLF (1187-1531 aa encoded by BC140791)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.53 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP ( TOP2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 24641-1-AP (TOP2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 24641-1-AP (TOP2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.