F55028

antibody from NSJ Bioreagents

Targeting: TOP2A TOP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55028

Provider

NSJ Bioreagents

Product name

Topoisomerase II alpha Antibody / TOP2A

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Rabbit

Vial size

0.08 µl, 0.4 µl

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) Jurkat and 2) K562 cell lysate with Topoisomerase II alpha antibody. Predicted molecular weight ~174 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human A375 cell lysate with Topoisomerase II alpha antibody. Predicted molecular weight ~174 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human HeLa cells with Topoisomerase II alpha antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skin tissue with Topoisomerase II alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.